HST

Gold Taq Hot-Start Polymerase

£329.00£1,484.00

The hot-start polymerase used in MegaMix Gold but as a separate enzyme and buffer.

Microzone’s Gold Taq Hot-Start Polymerase provides enhanced sensitivity and specificity for complex PCR applications, such as high-resolution melting, multiplex PCR, and genotyping. This enzyme remains inactive at room temperature, preventing non-specific amplification, and is activated by heating during the PCR cycling. Optimized for robust performance in demanding conditions, Gold Taq Hot-Start Polymerase supports amplifications up to 6 kb and is compatible with rapid cycling protocols. Supplied with an optimized reaction buffer, this enzyme ensures precise and consistent results for research-grade applications.

  • High Specificity: Prevents non-specific amplification and primer-dimer formation.
  • Broad Applicability: Suitable for fast PCR, multiplex PCR, and GC-rich templates.
  • Stability: Withstands multiple freeze-thaw cycles.
  • Optimized Buffer: Enhances amplification of complex sequences
  • Hot Start PCR (up to 6 kb)
  • qPCR (probe- or dye-based)
  • Fast and Multiplex PCR
  • Genotyping
  • Amplification of GC-/AT-rich templates
  • TA Cloning
  • High-Resolution Melting
  • Concentration: 5 U/µL in storage buffer.
  • Buffer: 5X Reaction Buffer with 200 µM dNTPs, 3 mM MgCl₂.
  • Formats: Available in 500- and 2500-unit options.
  • Storage: Maintain at recommended conditions to ensure quality.

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