MegaMix Emerald qPCR Mastermix (Dye Based)
£101.00 – £450.00
Everything you need for efficient qPCR in a single reaction, swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye (microGREEN), dUTP and UNG in enhancing buffer, optimised for amplifying low-copy DNA targets. The third-generation intercalating fluorescent dye (microGREEN), binds to double-stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and is ideal for use with MegaMix HRM (High Resolution Melting).
Everything you need for efficient qPCR in a single reaction, swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye (microGREEN), dUTP and UNG in enhancing buffer, optimised for amplifying low-copy DNA targets. The third-generation intercalating fluorescent dye (microGREEN), binds to double-stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and is ideal for use with MegaMix HRM (High Resolution Melting).
High success rates up to 6kb.
High yields under standard conditions.
Provides effective and efficient amplification for GC and AT rich templates.
Strong MgCl2 to support strong amplification.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
Can be used with standard and fast cycling and amplification.
Works well with dye based assays.
PCR water supplied with kit
Comes with dUTP and UNG
Dye based qPCR
Use with gDNA, Viral DNA, cDNA….
Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
Why Choose Clent
We strive to enable our customers to focus on the results of their work, rather than the tools they use. Need to speak with a member of our team? Get in touch today.
