MegaMix HRM Mastermix
£90.00 – £750.00
MegaMix HRM – High Resolution Melting PCR Mastermix
MegaMix HRM - High Resolution Melting PCR Mastermix
MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating theformation of non-specific amplification and primer-dimers.
This mastermix performs excellently when amplifying GC rich templates, providing reproducible and reliable results with little or no optimisation.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating
dye, microGREEN, at a saturating concentration ideal for the most sensitive of HRM
applications.
High Resolution Melt (HRM) analysis:
Single Nucleotide Polymorphism (SNP) genotyping
Methylation analysis
Mutation scanning
Detection of sequence variations
Available in the following formats:
1mL
5 x 1mL
10 x 1mL
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