2MM-HRM-1

MegaMix HRM Mastermix

£90.00£750.00

MegaMix HRM – High Resolution Melting PCR Mastermix

MegaMix HRM - High Resolution Melting PCR Mastermix

MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.

The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating theformation of non-specific amplification and primer-dimers.

This mastermix performs excellently when amplifying GC rich templates, providing reproducible and reliable results with little or no optimisation.

MegaMix - by Microzone

Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:

  • Routine End Point PCR
  • Hot Start End Point PCR
  • qPCR
  • RT-qPCR
  • High Fidelity (HiFi) PCR
  • Inhibitor Resistant PCR
  • High Resolution Melting
  • RT-PCR

Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......

Request Sample

    MegaMix HRM - High Resolution Melting PCR Mastermix

    MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.

    The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating theformation of non-specific amplification and primer-dimers.

    This mastermix performs excellently when amplifying GC rich templates, providing reproducible and reliable results with little or no optimisation.

    MegaMix - by Microzone

    Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:

    • Routine End Point PCR
    • Hot Start End Point PCR
    • qPCR
    • RT-qPCR
    • High Fidelity (HiFi) PCR
    • Inhibitor Resistant PCR
    • High Resolution Melting
    • RT-PCR

    Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......

    Request Sample

    • Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating
      dye, microGREEN, at a saturating concentration ideal for the most sensitive of HRM
      applications.

    High Resolution Melt (HRM) analysis:

    Single Nucleotide Polymorphism (SNP) genotyping
    Methylation analysis
    Mutation scanning
    Detection of sequence variations

    Available in the following formats:

    1mL

    5 x 1mL

    10 x 1mL

    • Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating
      dye, microGREEN, at a saturating concentration ideal for the most sensitive of HRM
      applications.

    High Resolution Melt (HRM) analysis:

    Single Nucleotide Polymorphism (SNP) genotyping
    Methylation analysis
    Mutation scanning
    Detection of sequence variations

    Available in the following formats:

    1mL

    5 x 1mL

    10 x 1mL

    Download essential technical information for this product.

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