MegaMix End Point PCR Mastermix:
Microzone's first MegaMixes. Over the years this MegaMix has stood the test of timel. It's still a firm favourite with many customers continually coming back to order again and again.
From the start we knew that the first few cycles of PCR are always the most important to successful amplification. Therefore, our mastermixes are never short of enough polymerase to kick start and then powerfully continue your amplification. MegaMix (and MegaMix Blue) are designed to cope with whatever is thrown at them. We incorporate two different Taq polymerases. The two Taqs complement each other and offer customers the capability to cover a large range of templates with just one mastermix. The addition of Microzone’s own proprietary engineered PCR buffer ensures that our mastermixes provide powerful, broad template, amplification.
Looking for a non-hot start PCR mastermix? You can't go far wrong with our tried and fully tested over many years MegaMix or MegaMix Blue PCR mastermixes.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Specially formulated Taq polymerase blend in optimised buffer for efficient PCR
amplification. - Optimised buffer for effective and efficient amplification for tough GC and AT rich
templates. - Available as MegaMix (1x Conc) and MegaMix Double (2x Conc)
Routine end point PCR.
Fast PCR.
Amplification of GC and AT rich templates.
Up to 6kb.
Methylated DNA.
TA Cloning
Cycling Profile (guide only)
Initial denaturation step: 95⁰C for 3 mins
Then Cycle 25-30 times:
Step 1: 95⁰C for 30 secs
Step 2: Optimal annealing temp. of primers for 30 to 60 secs
Step 3: 72⁰C for 45 to 60 secs
Cool to room temperature
Thaw reagents prior to use. Mix well prior to use.
GC and complex templates may require higher denaturation temperatures
Two step cycling protocols can increase speed. Following annealing just ramp the temperature back to denaturation.
If using new primers or changing your master mix it will be necessary to perform a new annealing temperature gradient. We suggest 1⁰C intervals and to start at 5⁰C below the calculated Tm of the primers and go to 5⁰C above the primer Tm.
Primers design and quality have a large impact on the reaction. Taking time to design your primers using good techniques or software will benefit down the line. We do offer a technical note on primer design. Contact us to receive a copy.
Why Choose Clent
We strive to enable our customers to focus on the results of their work, rather than the tools they use. Need to speak with a member of our team? Get in touch today.
