MegaMix Platinum Probe RT-qPCR Kit
£97.00 – £800.00
MegaMix Platinum Probe RT-qPCR Kit with ready to use mastermixes
MegaMix Platinum Probe RT-qPCR Kit
Containing all the components needed to perform multiplex probe RT-qPCR swiftly and reliably in one reaction. This mix has been optimised
- for multiplex assays utilising dual labelled hydrolysis probes.
- to be compatible with both standard and fast cycling conditions.
- with incorporation of thermolabile UNG and dUTP, this system eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
Our One-Step RT-qPCR probe mix contains a fast acting and stable Reverse Transcriptase coupled with our MegaMix Platinum (a high performing mastermix that works with numerous RNA templates including GC/AT rich and in both standard and fast thermocyclers).
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
MegaMix Platinum 2x One-Step RT-qPCR with UNG kit shows excellent linearity and reproduciility with 6x 1 in 10 dilution series of a high concentration sample. SARs-CoV-2 E gene, BMS MIC
Linear dynamic range for E gene detection from SARS-CoV-2 using MegaMixPlatinum Probe RT-qPCR kit. Seven 10-fold dilution of SARS-CoV-2 RNA was performed to demonstrate dynamic range using specific primers to detect E gene. Inset numbers show the average Quantification Cycle (Cq) of 7 replicates per each dilution evaluated. Data are presented as mean + SEM.
MegaMix Platinum Probe RT-qPCR Kit
Containing all the components needed to perform multiplex probe RT-qPCR swiftly and reliably in one reaction. This mix has been optimised
- for multiplex assays utilising dual labelled hydrolysis probes.
- to be compatible with both standard and fast cycling conditions.
- with incorporation of thermolabile UNG and dUTP, this system eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
Our One-Step RT-qPCR probe mix contains a fast acting and stable Reverse Transcriptase coupled with our MegaMix Platinum (a high performing mastermix that works with numerous RNA templates including GC/AT rich and in both standard and fast thermocyclers).
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Robust, linear, one-step RT-qPCR amplification of multiple RNA targets for probe
based detection. - Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications. - One-Step format slashes time and effort required to get reliable results.
- RT-qPCR RNA based assays using probes such as TAQ Man, Scorpion Probes etc.
- Viral RNA assays -eg SARS-CoV-2 assays.
- Quantification of RNA, gDNA, cDNA.
- Multiplex assay development.
. The kit consists of
- 2X MegaMix Platinum Probe qPCR mastermix, containing chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in enhancing buffer;
- 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets; and
- Just Water, our molecular biology grade water.
Available in 100, 200 & 1000 reactions.
- Robust, linear, one-step RT-qPCR amplification of multiple RNA targets for probe
based detection. - Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications. - One-Step format slashes time and effort required to get reliable results.
- RT-qPCR RNA based assays using probes such as TAQ Man, Scorpion Probes etc.
- Viral RNA assays -eg SARS-CoV-2 assays.
- Quantification of RNA, gDNA, cDNA.
- Multiplex assay development.
. The kit consists of
- 2X MegaMix Platinum Probe qPCR mastermix, containing chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in enhancing buffer;
- 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets; and
- Just Water, our molecular biology grade water.
Available in 100, 200 & 1000 reactions.
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