MegaMix Platinum Probe RT-qPCR Kit
Containing all the components needed to perform multiplex probe RT-qPCR swiftly and reliably in one reaction. This mix has been optimised
- for multiplex assays utilising dual labelled hydrolysis probes.
- to be compatible with both standard and fast cycling conditions.
- with incorporation of thermolabile UNG and dUTP, this system eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis.
Our One-Step RT-qPCR probe mix contains a fast acting and stable Reverse Transcriptase coupled with our MegaMix Platinum (a high performing mastermix that works with numerous RNA templates including GC/AT rich and in both standard and fast thermocyclers).
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Robust, linear, one-step RT-qPCR amplification of multiple RNA targets for probe
based detection. - Confidence inspiring, incorporation of thermolabile UNG and dUTP’s prevent
carryover contamination and allow for use in downstream applications. - One-Step format slashes time and effort required to get reliable results.
- RT-qPCR RNA based assays using probes such as TAQ Man, Scorpion Probes etc.
- Viral RNA assays -eg SARS-CoV-2 assays.
- Quantification of RNA, gDNA, cDNA.
- Multiplex assay development.
. The kit consists of
- 2X MegaMix Platinum Probe qPCR mastermix, containing chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in enhancing buffer;
- 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets; and
- Just Water, our molecular biology grade water.
Available in 100, 200 & 1000 reactions.
Why Choose Clent
We strive to enable our customers to focus on the results of their work, rather than the tools they use. Need to speak with a member of our team? Get in touch today.
