BCHM-ASY

LCGreen Plus

£150.00£850.00

LCGreen Plus is a fluorescent dye specifically formulated for High-Resolution Melting (HRM) curve analysis, enabling the detection of DNA sequence variants such as mutations and polymorphisms. Compatible with various fluorescence-based PCR detection systems like the Roche LightCycler®, it excels when used with high-resolution melting instruments. Manufactured exclusively by BioFire, LCGreen Plus stands out for its unique chemical structures and superb fluorescence intensity.

LCGreen Plus is a fluorescent dye specifically formulated for High-Resolution Melting (HRM) curve analysis, enabling the detection of DNA sequence variants such as mutations and polymorphisms. Compatible with various fluorescence-based PCR detection systems like the Roche LightCycler®, it excels when used with high-resolution melting instruments. Manufactured exclusively by BioFire, LCGreen Plus stands out for its unique chemical structures and superb fluorescence intensity.

  • Optimized for HRM Analysis: Designed to detect DNA sequence variants accurately.
  • High Fluorescence Intensity: Ensures clear visibility and distinction in HRM curve analysis.
  • Broad Instrument Compatibility: Can be used with Roche LightCycler® and other fluorescence-based PCR systems.
  • Enhanced Detection: Superior fluorescence intensity enhances the detection of mutations and polymorphisms.
  • Increased DNA Melting Temperature: Adds 1 – 3 °C to the DNA melting temperature, improving differentiation of closely related DNA sequences.
  • Stable and Reliable: Offers consistent performance with a stability of one year at –20 °C and up to 6 months at 4 °C after first use.

DNA Sequence Variant Detection: Ideal for identifying mutations, polymorphisms, and other DNA sequence variants through HRM curve analysis in genetic research and diagnostics.

  • Optimum Excitation/Emission: 440 – 470 nm (excitation) and 470 – 520 nm (emission), varying with solution conditions.
  • Storage: Shipped at ambient temperature, stored at –20 °C upon receipt, and 4 °C after first use.
  • Shelf Life: One year at –20 °C, six months at 4 °C.
  • Adjustments: May require cycling parameter adjustments due to its effect on DNA melting temperatures.

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