MegaMix Emerald qPCR Mastermix (Dye Based)
£56.00 – £450.00
MegaMix Emerald – dye based qPCR mastermix
MegaMix Emerald dye based qPCR Mastermix
Containing all the components needed to perform dye based qPCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing Microzone's proprietary PCR buffer optimised for amplifying low copy DNA targets.
We have incorporated MicroGREEN Dye ( a third generation intercalating highly fluorescent dye that binds to double stranded DNA) in to MegaMix Emerald. This dye mix combinations makes MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High Resolution Melting (HRM).
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.
MegaMix Emerald using MicroGreen dye ensures high sensitivity and earlier CTs than competitor mixes. When combined with Microzone's SwiftScript cDNA synthesis product, you achieve the perfect combination for RT-qPCR too.
The product is stable at -20°C, 4°C and RT for various time lengths.
MegaMix - by Microzone
Microzone's MegaMix range of PCR mastermixes covers a broad range of a scientist's requirements. We offer a MegaMix for most occasions including:
- Routine End Point PCR
- Hot Start End Point PCR
- qPCR
- RT-qPCR
- High Fidelity (HiFi) PCR
- Inhibitor Resistant PCR
- High Resolution Melting
- RT-PCR
Associated products include - Taq's with buffer; dNTP mixes; Molecular Grade Water.......
- Incorporates Microzone’s proprietary third generation, non-inhibitory intercalating dye, microGREEN, for increased fluorescence and unmatched sensitivity.
- Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
- Compatible with any qPCR instrument with a FAM/SYBR channel.
- Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—Single copy detection.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-Plus and microLYSIS-RNA.
- Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
Dye based qPCR
Use with gDNA, Viral DNA, cDNA….
Use for quantification, gene expression, SNP detection using High Resolution Melting (HRM).
MegaMix Emerald qPCR with UNG (orange) exhibits earlier Cq values and superior sensitivity vs competitor A (black), when amplifying the RNase P gene from a 6X, 1 in 10 serial dilution of 1 µg human DNA. BMS MIC.
Why Choose Clent
We strive to enable our customers to focus on the results of their work, rather than the tools they use. Need to speak with a member of our team? Get in touch today.
