High-fidelity DNA Polymerases & When to use them
Taq polymerase is the tried and true standard when it comes to PCR, but that doesn’t mean it’s the most accurate polymerase for high-sensitivity applications. Instead, alternative enzymes, known as high-fidelity polymerases, offer higher accuracies in the amplicons resulting from PCR thanks to a combination of more stringent base reading and proofreading activities. While you … Read more